linear gradient sds page mini gel Search Results


sds-page 5–20 % gradient gel  (FUJIFILM)
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FUJIFILM sds-page 5–20 % gradient gel
Sds Page 5–20 % Gradient Gel, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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4 to 20% sds-page gels  (NuSep Inc)
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NuSep Inc 4 to 20% sds-page gels
4 To 20% Sds Page Gels, supplied by NuSep Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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longlife 4–20% gradient tris-hepes-sds page mini gel  (Gradipore Inc)
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Gradipore Inc longlife 4–20% gradient tris-hepes-sds page mini gel
Longlife 4–20% Gradient Tris Hepes Sds Page Mini Gel, supplied by Gradipore Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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8-16% gradient sds-page gel (serva lot v140115-1)  (SERVA Electrophoresis)
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SERVA Electrophoresis 8-16% gradient sds-page gel (serva lot v140115-1)
8 16% Gradient Sds Page Gel (Serva Lot V140115 1), supplied by SERVA Electrophoresis, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
8-16% gradient sds-page gel (serva lot v140115-1) - by Bioz Stars, 2026-10
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4-20% pre-casted gradient sds page gel  (BioExpress)
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BioExpress 4-20% pre-casted gradient sds page gel
Expression of MsSCP-x and MsSCP-2 . (A) The semi-quantitative RT-PCR shows that mRNA levels from different tissues at different developmental stages of M. sexta . Lane A: Day 1-4 th instar; lane B: Day 3-4 th instar; lane C: Day 1-5 th instar; lane D: Day 4-5 th instar; lane E: Day 1-wandering stage; lane F: Day 1-pupal stage. The Manduca rpS3 gene was used as internal standard for the RT-PCR. Only 30 cycles of PCR were performed to avoid saturating the signals. (B). Proteins from different tissues of Day 3-4 th instar M. sexta larva were loaded 20 μg per lane except lane 8 that only had 10 ng purified recombinant MsSCP-2. Protein was analyzed on 4-20% gradient <t>SDS</t> <t>PAGE.</t> Lane 1: protein size marker; Lane 2: hemolymph; Lane 3: midgut; Lane 4: hindgut; Lane 5: fat body; Lane 6: muscle; Lane 7: epidermis; Lane 8: recombinant MsSCP-2.
4 20% Pre Casted Gradient Sds Page Gel, supplied by BioExpress, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
4-20% pre-casted gradient sds page gel - by Bioz Stars, 2026-10
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7.0% - 20% gradient pre-cast polyacrylamide sds-page gel  (Jule Inc)
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Jule Inc 7.0% - 20% gradient pre-cast polyacrylamide sds-page gel
Expression of MsSCP-x and MsSCP-2 . (A) The semi-quantitative RT-PCR shows that mRNA levels from different tissues at different developmental stages of M. sexta . Lane A: Day 1-4 th instar; lane B: Day 3-4 th instar; lane C: Day 1-5 th instar; lane D: Day 4-5 th instar; lane E: Day 1-wandering stage; lane F: Day 1-pupal stage. The Manduca rpS3 gene was used as internal standard for the RT-PCR. Only 30 cycles of PCR were performed to avoid saturating the signals. (B). Proteins from different tissues of Day 3-4 th instar M. sexta larva were loaded 20 μg per lane except lane 8 that only had 10 ng purified recombinant MsSCP-2. Protein was analyzed on 4-20% gradient <t>SDS</t> <t>PAGE.</t> Lane 1: protein size marker; Lane 2: hemolymph; Lane 3: midgut; Lane 4: hindgut; Lane 5: fat body; Lane 6: muscle; Lane 7: epidermis; Lane 8: recombinant MsSCP-2.
7.0% 20% Gradient Pre Cast Polyacrylamide Sds Page Gel, supplied by Jule Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
7.0% - 20% gradient pre-cast polyacrylamide sds-page gel - by Bioz Stars, 2026-10
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4-20% gradient polyacrylamide sds-page gel  (Shanghai WSHT Biotechnology Inc)
90
Shanghai WSHT Biotechnology Inc 4-20% gradient polyacrylamide sds-page gel
Expression of MsSCP-x and MsSCP-2 . (A) The semi-quantitative RT-PCR shows that mRNA levels from different tissues at different developmental stages of M. sexta . Lane A: Day 1-4 th instar; lane B: Day 3-4 th instar; lane C: Day 1-5 th instar; lane D: Day 4-5 th instar; lane E: Day 1-wandering stage; lane F: Day 1-pupal stage. The Manduca rpS3 gene was used as internal standard for the RT-PCR. Only 30 cycles of PCR were performed to avoid saturating the signals. (B). Proteins from different tissues of Day 3-4 th instar M. sexta larva were loaded 20 μg per lane except lane 8 that only had 10 ng purified recombinant MsSCP-2. Protein was analyzed on 4-20% gradient <t>SDS</t> <t>PAGE.</t> Lane 1: protein size marker; Lane 2: hemolymph; Lane 3: midgut; Lane 4: hindgut; Lane 5: fat body; Lane 6: muscle; Lane 7: epidermis; Lane 8: recombinant MsSCP-2.
4 20% Gradient Polyacrylamide Sds Page Gel, supplied by Shanghai WSHT Biotechnology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
4-20% gradient polyacrylamide sds-page gel - by Bioz Stars, 2026-10
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sds-page 4-20% gradient gel  (PAGEgel Inc)
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PAGEgel Inc sds-page 4-20% gradient gel
Expression of MsSCP-x and MsSCP-2 . (A) The semi-quantitative RT-PCR shows that mRNA levels from different tissues at different developmental stages of M. sexta . Lane A: Day 1-4 th instar; lane B: Day 3-4 th instar; lane C: Day 1-5 th instar; lane D: Day 4-5 th instar; lane E: Day 1-wandering stage; lane F: Day 1-pupal stage. The Manduca rpS3 gene was used as internal standard for the RT-PCR. Only 30 cycles of PCR were performed to avoid saturating the signals. (B). Proteins from different tissues of Day 3-4 th instar M. sexta larva were loaded 20 μg per lane except lane 8 that only had 10 ng purified recombinant MsSCP-2. Protein was analyzed on 4-20% gradient <t>SDS</t> <t>PAGE.</t> Lane 1: protein size marker; Lane 2: hemolymph; Lane 3: midgut; Lane 4: hindgut; Lane 5: fat body; Lane 6: muscle; Lane 7: epidermis; Lane 8: recombinant MsSCP-2.
Sds Page 4 20% Gradient Gel, supplied by PAGEgel Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
sds-page 4-20% gradient gel - by Bioz Stars, 2026-10
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5e20% gradient pre-cast gel for sds-page  (ATTO Technology Inc)
90
ATTO Technology Inc 5e20% gradient pre-cast gel for sds-page
Expression of MsSCP-x and MsSCP-2 . (A) The semi-quantitative RT-PCR shows that mRNA levels from different tissues at different developmental stages of M. sexta . Lane A: Day 1-4 th instar; lane B: Day 3-4 th instar; lane C: Day 1-5 th instar; lane D: Day 4-5 th instar; lane E: Day 1-wandering stage; lane F: Day 1-pupal stage. The Manduca rpS3 gene was used as internal standard for the RT-PCR. Only 30 cycles of PCR were performed to avoid saturating the signals. (B). Proteins from different tissues of Day 3-4 th instar M. sexta larva were loaded 20 μg per lane except lane 8 that only had 10 ng purified recombinant MsSCP-2. Protein was analyzed on 4-20% gradient <t>SDS</t> <t>PAGE.</t> Lane 1: protein size marker; Lane 2: hemolymph; Lane 3: midgut; Lane 4: hindgut; Lane 5: fat body; Lane 6: muscle; Lane 7: epidermis; Lane 8: recombinant MsSCP-2.
5e20% Gradient Pre Cast Gel For Sds Page, supplied by ATTO Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
5e20% gradient pre-cast gel for sds-page - by Bioz Stars, 2026-10
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pre-cast 15–40% nonreducing gradient sds-page gel  (Gradipore Inc)
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Gradipore Inc pre-cast 15–40% nonreducing gradient sds-page gel
Expression of MsSCP-x and MsSCP-2 . (A) The semi-quantitative RT-PCR shows that mRNA levels from different tissues at different developmental stages of M. sexta . Lane A: Day 1-4 th instar; lane B: Day 3-4 th instar; lane C: Day 1-5 th instar; lane D: Day 4-5 th instar; lane E: Day 1-wandering stage; lane F: Day 1-pupal stage. The Manduca rpS3 gene was used as internal standard for the RT-PCR. Only 30 cycles of PCR were performed to avoid saturating the signals. (B). Proteins from different tissues of Day 3-4 th instar M. sexta larva were loaded 20 μg per lane except lane 8 that only had 10 ng purified recombinant MsSCP-2. Protein was analyzed on 4-20% gradient <t>SDS</t> <t>PAGE.</t> Lane 1: protein size marker; Lane 2: hemolymph; Lane 3: midgut; Lane 4: hindgut; Lane 5: fat body; Lane 6: muscle; Lane 7: epidermis; Lane 8: recombinant MsSCP-2.
Pre Cast 15–40% Nonreducing Gradient Sds Page Gel, supplied by Gradipore Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
pre-cast 15–40% nonreducing gradient sds-page gel - by Bioz Stars, 2026-10
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gradient gel sds-page superseptmace 10-20  (FUJIFILM)
90
FUJIFILM gradient gel sds-page superseptmace 10-20
Expression of MsSCP-x and MsSCP-2 . (A) The semi-quantitative RT-PCR shows that mRNA levels from different tissues at different developmental stages of M. sexta . Lane A: Day 1-4 th instar; lane B: Day 3-4 th instar; lane C: Day 1-5 th instar; lane D: Day 4-5 th instar; lane E: Day 1-wandering stage; lane F: Day 1-pupal stage. The Manduca rpS3 gene was used as internal standard for the RT-PCR. Only 30 cycles of PCR were performed to avoid saturating the signals. (B). Proteins from different tissues of Day 3-4 th instar M. sexta larva were loaded 20 μg per lane except lane 8 that only had 10 ng purified recombinant MsSCP-2. Protein was analyzed on 4-20% gradient <t>SDS</t> <t>PAGE.</t> Lane 1: protein size marker; Lane 2: hemolymph; Lane 3: midgut; Lane 4: hindgut; Lane 5: fat body; Lane 6: muscle; Lane 7: epidermis; Lane 8: recombinant MsSCP-2.
Gradient Gel Sds Page Superseptmace 10 20, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
gradient gel sds-page superseptmace 10-20 - by Bioz Stars, 2026-10
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sephacryl 400 hr premade 4–15% gradient polyacrylamide phast gel for sds-page  (Pharmacia LKB Biotechnology Inc)
90
Pharmacia LKB Biotechnology Inc sephacryl 400 hr premade 4–15% gradient polyacrylamide phast gel for sds-page
Expression of MsSCP-x and MsSCP-2 . (A) The semi-quantitative RT-PCR shows that mRNA levels from different tissues at different developmental stages of M. sexta . Lane A: Day 1-4 th instar; lane B: Day 3-4 th instar; lane C: Day 1-5 th instar; lane D: Day 4-5 th instar; lane E: Day 1-wandering stage; lane F: Day 1-pupal stage. The Manduca rpS3 gene was used as internal standard for the RT-PCR. Only 30 cycles of PCR were performed to avoid saturating the signals. (B). Proteins from different tissues of Day 3-4 th instar M. sexta larva were loaded 20 μg per lane except lane 8 that only had 10 ng purified recombinant MsSCP-2. Protein was analyzed on 4-20% gradient <t>SDS</t> <t>PAGE.</t> Lane 1: protein size marker; Lane 2: hemolymph; Lane 3: midgut; Lane 4: hindgut; Lane 5: fat body; Lane 6: muscle; Lane 7: epidermis; Lane 8: recombinant MsSCP-2.
Sephacryl 400 Hr Premade 4–15% Gradient Polyacrylamide Phast Gel For Sds Page, supplied by Pharmacia LKB Biotechnology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Expression of MsSCP-x and MsSCP-2 . (A) The semi-quantitative RT-PCR shows that mRNA levels from different tissues at different developmental stages of M. sexta . Lane A: Day 1-4 th instar; lane B: Day 3-4 th instar; lane C: Day 1-5 th instar; lane D: Day 4-5 th instar; lane E: Day 1-wandering stage; lane F: Day 1-pupal stage. The Manduca rpS3 gene was used as internal standard for the RT-PCR. Only 30 cycles of PCR were performed to avoid saturating the signals. (B). Proteins from different tissues of Day 3-4 th instar M. sexta larva were loaded 20 μg per lane except lane 8 that only had 10 ng purified recombinant MsSCP-2. Protein was analyzed on 4-20% gradient SDS PAGE. Lane 1: protein size marker; Lane 2: hemolymph; Lane 3: midgut; Lane 4: hindgut; Lane 5: fat body; Lane 6: muscle; Lane 7: epidermis; Lane 8: recombinant MsSCP-2.

Journal: BMC Physiology

Article Title: Sterol carrier protein-x gene and effects of sterol carrier protein-2 inhibitors on lipid uptake in Manduca sexta

doi: 10.1186/1472-6793-10-9

Figure Lengend Snippet: Expression of MsSCP-x and MsSCP-2 . (A) The semi-quantitative RT-PCR shows that mRNA levels from different tissues at different developmental stages of M. sexta . Lane A: Day 1-4 th instar; lane B: Day 3-4 th instar; lane C: Day 1-5 th instar; lane D: Day 4-5 th instar; lane E: Day 1-wandering stage; lane F: Day 1-pupal stage. The Manduca rpS3 gene was used as internal standard for the RT-PCR. Only 30 cycles of PCR were performed to avoid saturating the signals. (B). Proteins from different tissues of Day 3-4 th instar M. sexta larva were loaded 20 μg per lane except lane 8 that only had 10 ng purified recombinant MsSCP-2. Protein was analyzed on 4-20% gradient SDS PAGE. Lane 1: protein size marker; Lane 2: hemolymph; Lane 3: midgut; Lane 4: hindgut; Lane 5: fat body; Lane 6: muscle; Lane 7: epidermis; Lane 8: recombinant MsSCP-2.

Article Snippet: Proteins (20 μg) from each sample were resolved on a 4-20% pre-casted gradient SDS PAGE gel (ISC Bioexpress, Kaysville, UT, USA) and transferred onto a Hybond-C extra membrane (Amersham BioScience, Piscataway, NJ, USA) at 18 volts overnight in Tris-glycine transfer buffer (0.303% Tris base, 1.44% glycine, 20% methanol) as described [ ].

Techniques: Expressing, Quantitative RT-PCR, Reverse Transcription Polymerase Chain Reaction, Purification, Recombinant, SDS Page, Marker